In line with the immunoblots, quantification by flow cytometry indicated accumulation of autophagosomes upon infection in both wild-type and Usp25−/− cells, with a significantly higher population of non-degradative autophagosomes in the Usp25−/− cells for both mock and infected cells (Figures 3D, 3E, and S3A). The gene discussed is USP25; the disease is infection.