We then draw the Kaplan–Meier (K-M) curve of tumors which significantly affected the OS time and cancer prognosis, and further demonstrate that TCGA-GMBLGG, TCGA-KIRC, TCGA-SKCM, and TARGET-ALL had worse prognosis with low-ROCK1 expression while the high expression of ROCK1 in TCGA-LGG, TARGET-LAML, and TARGET-NB was correlated to the low survival probability (Supplementary Figure 4). The gene discussed is ROCK1; the disease is neuroblastoma.