Similarly, preoperative serum CA125 showed remarkable predictive value of the prognosis in the subgroup of ICC patients with TNM stage III to IV, tumor size ≤ 5 cm, tumor size > 5 cm, well/moderate differentiation, poor differentiation, single-tumor, no lymph node metastasis, R0 surgical resection margin, no tumor capsule, HBsAg negative status, Child–Pugh class A and CA19-9 > 35.0 U/mL (all P < .05) (Figs. 3B–F, 4A–D, and 5A–C). This evidence concerns the gene MUC16 and neoplasm.