Furthermore, in subgroups of ICC patients with TNM stage III to IV, tumor size ≤ 5 cm, tumor size > 5 cm, well/moderate differentiation, poor differentiation, single-tumor, no lymph node metastasis, no capsule, serum HBsAg negativity, Child–Pugh Class A and an elevated serum CA19-9 level, preoperative elevated CA125 also showed value as a predictor of poorer OS and DFS. The gene discussed is MUC16; the disease is neoplasm.