We first measured the levels of TGFβ1 by enzyme-linked immunosorbent assay in the SSc serum samples that were used to stimulate HdLy-MVECs in the in vitro assays, and found that they were significantly increased (mean ± SEM, 91.39 ± 17.32 pg/mL) compared to serum samples from healthy donors (mean ± SEM, 36.06 ± 10.28 pg/mL; p = 0.02). The gene discussed is TGFB1; the disease is systemic sclerosis.