TBX1 and cancer: Since CAFs are greatly influenced by neighboring malignant tumors and, in fact, it has been suggested that normal fibroblasts could be converted into CAFs within the tumor microenvironment via various mechanisms [39], we rationally speculated that the use of 3D spheroid cultures in conjunction with cellular metabolic measurements might provide additional insights into strategies for the identification and characterizations of various CAFs that originate from different malignant tumors.