Nonobese mice with hepatocyte-specific FASN deficiency (HKO mice) that were fed a zero-fat diet (ZFD) containing a high proportion of sucrose (62% by weight), but not those fed normal chow, developed aggravated NAFLD (despite impairment of DNL) as well as hypoglycemia due to impaired FAO (30). The gene discussed is FASN; the disease is metabolic dysfunction-associated steatotic liver disease.