Initially, we determined the basal mRNA levels of NORAD in a set of human breast cancer cell lines (MCF-7, MDA-MB-231, -436, and -468) and in a non-malignant human mammary epithelial cell line (MCF-10A) by real-time quantitative reverse transcriptase polymerase chain reaction (qRT-PCR). The gene discussed is NORAD; the disease is breast cancer.