The FACS analysis showed that the number of Ly6C+CX3CR1+ IMs showed no difference between the two groups, but the Ly6CloCX3CR1+ IMs decreased obviously in myeloid-specific RBP-J–deficient mice (Figures 3D–F), suggesting that RBP-J deficiency in myeloid cells could mainly affect the differentiation of Ly6Clo IMs during PD progression. The gene discussed is CX3CR1; the disease is Parkinson disease.