Still, reports on how these therapies affect the immune system and modulate the TME are scarce in the human medical literature, and in some cases suggest adaptation by neoplasms to upregulate the expression of PD-L1 after treatment.56-58 The second cohort evaluated in this study were archived pre- and post-treatment tissue sections from a clinical trial of histotripsy in ten canine patients with STS. This evidence concerns the gene CD274 and neoplasm.