To determine if ESRRG directly induces PKM expression in ESCC cells, we co-transfected the PKM promoter constructs (PKM-WT-Luc, PKM-MT1-Luc, PKM-MT2-Luc) with the ESRRG expression plasmid into 293 T and TE1 cells (Fig. 4F). This evidence concerns the gene PKM and esophageal squamous cell carcinoma.