The methylation levels of MYL1, MYH2 and MYH7 were significantly down-regulated (P<0.01) (Fig. 4A, D, E), while the methylation level of MYL2 (P>0.01), MYL3 (normal median=0.643, tumor median=0.642, P<0.01) and MYH10 (P>0.01) showed no significant changes in HNSCC tissues compared with normal tissues (Fig. 4B, C, F). The gene discussed is MYL1; the disease is head and neck squamous cell carcinoma.