Subsequently, the stepwise logistic analysis showed that the independent risk factors for LNM in CRC were histologic grade (G3) (OR =2.60, p =0.043), histologic grade (mucin-producing subtype) (OR =3.38, p =0.032), LVI (2.42, p <0.01), CEA level(>0.5ng/ml) (OR =1.85, p =0.042) and Man (high) (OR =2.65, p =0.006). The gene discussed is CEACAM5; the disease is colorectal carcinoma.