Further investigation of genes reportedly upregulated in hyperplasia (Beck et al., 2022) revealed that Ki-67 (MKI67) expression is increased upon Der p 2 pep exposure in KC clusters basal 1 and basal 2, mitotic and hair follicle KC, and also in the suprabasal KC clusters granular 1 and granular 2 but not in spinous and sebaceous gland KCs (Figure 5D, upper panel). This evidence concerns the gene CALCA and keratoconus.