To observe neutrophils generated from infection-experienced or infection-naïve HSPCs, HSPCs were FACS-isolated from 3 dpf Tg(Runx1:EGFP;lyz:DsRED2) larvae 1 day following injection with 600 CFU Sal-GFP (infection-experienced) or PBS (infection-naïve) (Fig. 5C). Here, RUNX1 is linked to infection.