To validate the results of RIP-seq, the amounts of the RNAs in the RIP samples obtained from MDBK mock and MDBK 3 ́LTR AS1-S cells were determined using real-time RT-PCR; six genes included in the 4,607 genes in Fig. 5C and the GO term “Pathways in cancer” were selected, and GAPDH was also selected as a control gene (Fig. 6A). Here, GAPDH is linked to cancer.