The mRNA and protein levels of KRT5 significantly decreased after sh-KRT5 transfection and the cell viability (Figure 5d and e), colony formation (Figure 5f and g), and number of EDU positive cells (Figure 5h and i) in sh-TRIM29-treated SW480 and HCT-116 cells significantly increased, suggesting that KRT5 silencing neutralised the role of sh-TRIM29 in colon cancer cells. This evidence concerns the gene TRIM29 and malignant colon neoplasm.