SIK1 and neoplasm: The protocol of the in vivo experiments is shown in Figure 7A, and the images of the resected tumors of the HCT116shNC or HCT 116shSIK1 tumor-bearing nude mice are shown in Figure 7B. SIK1 knockdown significantly increased in vivo chemotherapy resistance, leading to decreased efficacy, tumor weight, and tumor volume in HCT116 nude mice (Figure 7B, C and D).