ROCK1 and Hyperglycemia: Additionally, in Rho-associated kinase 1 (ROCK1) transgenic mice, it was observed that hyperglycemia phosphorylated Drp1 serine residues via ROCK1, thereby promoting the recruitment of Drp1 into mitochondria and ultimately inducing mitochondrial fission, while knockdown of ROCK1 reduced glomerular apoptosis and mitochondrial ROS production, decreased proteinuria and reduced podocyte shedding (92).