To further evaluate PIK3CD-L and PIK3CD-S expression profiles in the in-vitro endocrine/solid tumor cell models, the RNA samples purified from breast cancer cell lines (MDA MB 231 and MCF-7), colon cancer cell lines (HT-29 and SW620), and lung cancer cell lines (A549 and H1299) were subjected to RT-PCR assays. The gene discussed is PIK3CD; the disease is malignant colon neoplasm.