Although we did not see dramatic immune cell infiltrations into the steatotic livers beyond the F4/80-positivity in immunohistology stains, but rather increased inflammatory chemokine expression levels together with hepatocyte damages and fat droplets, our study that has adopted HFD for 2 weeks could reveal TM4SF5-dependent features of earlier stages of NAFLD. The gene discussed is TM4SF5; the disease is metabolic dysfunction-associated steatotic liver disease.