GLA and Fabry disease: Female heterozygous FD mice (Gla-knockout mice, Gla+/−) were purchased from Cyagen (C57BL/6N-Glaem1Cya, KOCMP-11605-Gla, Suzhou, China); the male wild-type mice were mated with the female heterozygous FD mice to obtain male hemizygous FD mice to evaluate the therapeutic effect of AAV2/8-hGLA.