DNA sequencing and mutation analysis revealed high-frequency mutations of multiple liver cancer genes, including up-regulated genes such as MET and MYC and down-regulated genes such as PTEN, RB1, TP53 and CTNNB1, and multiple signal pathways, including p53 pathway, WNT pathway, NF-κB pathway, and TGF-β signal pathway and so on [45, 46]. This evidence concerns the gene TP53 and liver cancer.