The PLOD2 gene encodes a protein that plays a key role in tissue remodeling and fibrotic processes.<h4>Methods</h4>The m<sup>6</sup>A methylation and expression levels of PLOD2 were determined by m<sup>6</sup>A methylated RNA immunoprecipitation sequencing (MeRIP-seq) and MeRIP-quantitative polymerase chain reaction (qPCR) in the testes of varicocele rats compared with control. This evidence concerns the gene PLOD2 and varicocele.