SOD1 and amyotrophic lateral sclerosis: To shed light on the mystery of ALS caused by hSOD1 and C71G-hPFN1, in the present study, we first aimed to map out NMR dynamics of the conformational equilibrium on both ps-ns and μs-ms time scales, as well as thermodynamic stability of C71G-hPFN1 and nascent hSOD1.