The ER-negative breast cancer cell line BT-549 did not show a significantly high level of estrogen signaling (Fig. 7a) but demonstrated a high pro-inflammatory (TNF-α, IL-1α/−1β) and a low anti-inflammatory response (IL-4) relative to BT-483 and MCF-7 (Fig. 7b). This evidence concerns the gene IL4 and breast cancer.