By binding to MDM2, RG-7112 inhibits the interaction between p53 and MDM2, preventing the degradation of the p53 proteasome, which leads to stabilization and increased levels of the tumor suppressor protein p53 in the cell.369 In vitro experiments showed that RG-7112 can inhibit and kill SJSA-1 osteosarcoma cells with high expression of MDM2 protein, inducing dose-dependent cell cycle arrest in G1 and G2/M phases in HCT116 and SJSA1 cells. Here, TP53 is linked to osteosarcoma.