ANKRD1 and cardiac hypertrophy: For example, the Ankrd1 gene, a marker of cardiac hypertrophy that is markedly upregulated, had little or no detectable promoter H3K23Pr peak (Figure 5E), while its mRNA exhibited the highest fold increase after pressure overload, which was equivalent in the hearts of mice on BCAA control and BCAA-free diets (Figure 6E).