Our previous studies found that the quality of priming of SIY-reactive CD8+ T cells differs greatly between lung and flank KP.SIY TdLNs; priming of tumor-reactive CD8+ T cells in mediastinal TdLNs leads to significantly decreased effector T cell differentiation compared with priming in inguinal TdLNs draining flank tumors (8, 25). This evidence concerns the gene CD8A and neoplasm.