Osmotic pumps with Ang II were subcutaneously implanted into C57BL/6J mice (aged 8 weeks) to further elucidate the role of NKRF in CFs in pathological cardiac remodeling for 4 weeks to produce a pathological cardiac remodeling and fibrosis model of hypertension (Figure S2A, Supporting Information).[18] Picrosirius red (PSR) staining showed that the ECM of the myocardium had a significant increase in the collagen level in hypertensive mice (Figure S2B, Supporting Information). This evidence concerns the gene NKRF and hypertensive disorder.