In order to determine the inhibitory effect on de novo synthesized inflammatory mediators, including TNF-α (Fig. 4), IL-6 (Fig. 5), IL-8 (Fig. 6), and IL-4 (Fig. 7) released during an allergic reaction, the HMC-1 cells were pre-treated with KHs for 1 h and followed by a 24 h of induction with PMACI. Here, IL4 is linked to allergic disease.