To confirm whether PAR1CAR-T cells can specifically recognize and eliminate PAR1-expressing tumor cells, in vitro cytotoxicity assays were performed by incubating PAR1CAR-T cells with MIA PaCa-2, CFPAC-1, and HPAF-II cells at E/T ratios of 0.1, 1, 5, 10, and 20. Here, F2R is linked to neoplasm.