Subsequently, to further verify whether miR-370-3p/miR-136-5p participate in the process of ESCC disease by regulating MYO5A, we transfected OE19 and TE-1 with MYO5A overexpression vector and si-MYO5A (si-MYO5A1#, si-MYO5A2#) and examined the transfection ability using RT-qPCR. Here, MYO5A is linked to esophageal squamous cell carcinoma.