TP53 and neoplasm: To characterize tumor phylogenies and subclonal structures, we performed TARGET-seq analysis16, a technology that allows allelic-resolution genotyping, whole transcriptome and immunophenotypic analysis from the same single-cell, on 17517 Lin-CD34+ HSPCs from 14 TP53-sAML patients (Extended Data Fig. 1a), 9 age-matched healthy donors (HDs) and 8 previously published myelofibrosis (MF) patients (Fig. 1a, gating strategy shown in Extended Data Fig. 2a).