Our analysis revealed that the expression of most genes, including DNMT3B, NOP2, DNMT1, ALYREF, NSUN2, NSUN5, TET2, TET3, DNMT3A, TET1, and YBX1 were significantly higher in ESCC tissues than in normal adjacent tissues (Fig. 1A). The gene discussed is ALYREF; the disease is esophageal squamous cell carcinoma.