CD8A and neoplasm: After that, the tumor purity and immune scores of the three clusters were calculated by ESTIMATE analysis, and it was found that patients with low TLR expression had up-regulation of tumor purity and reduction of immune cell infiltration(including B cell, CD8+ T cell, Neutrophil, Macrophage, Myeloid dendritic cell, NK cell, etc.), which often results in dysregulation of the tumor's immune microenvironment and poor prognosis.