To determine whether ferroptosis was directly enhanced by increased abundance of PUFAs in CRTC3-KO HCC cells, assays involving the ACSL4 inhibitor (PRGL493) showed that CRTC3-WT and CRTC3-KO HCC cell death upon RSL3 treatment could be rescued by PRGL493, and the rescue degree was more significant in CRTC3-KO HCC cells (Fig. 4f). Here, CRTC3 is linked to hepatocellular carcinoma.