Initially, we performed differential expression analysis of 12 CRGs (ATP7B, CDKN2A, DLAT, DLD, FDX1, GLS, LIAS, LIPT1, MTF1, PDHA1, PDHB, SLC31A1) in four datasets (TCGA-ESCC, GSE20347, GSE38129, and ESCC datasets). This evidence concerns the gene ATP7B and esophageal squamous cell carcinoma.