Firstly, we collected RNA-seq data of 12 CRGs (ATP7B, CDKN2A, DLAT, DLD, FDX1, GLS, LIAS, LIPT1, MTF1, PDHA1, PDHB, SLC31A1) from the TCGA-ESCC dataset, GSE20347 dataset, GSE38129 dataset, and ESCC dataset. The gene discussed is DLAT; the disease is esophageal squamous cell carcinoma.