It was found that GPC3 was highly expressed in the tumor tissues compared with the non-HCC tissues according to The Cancer Genome Atlas (TCGA) cohort (n = 369), the RNA-seq (n = 65) and MS data (n = 152) of Mengchao Hepatobiliary Hospital of Fujian Medical University (Supplemental Figure S15). This evidence concerns the gene GPC3 and hepatocellular carcinoma.