To quantify cell apoptosis/necrosis mediated by G-NK cells, HepG2 cells and HeLa cells were coincubated with NK cells, R-NK cells or G-NK cells at the E/T ratio of 10:1, as indicated in Fig. 2A. The apoptosis/necrosis of the treated tumor cells was then analyzed by FCM after Annexin V-APC/propidium iodide (PI) staining. The gene discussed is ANXA5; the disease is neoplasm.