GLS and breast cancer: Therefore, we first conducted a pan-cancer analysis based on the transcriptomic data from the TCGA database, the result of which revealed that the expression of the GLS gene was significantly down-regulated in diverse cancer types, including but not limited to GBM, KICH, KIRC, and KIRP, etc. (Figure 2A), particularly in the breast cancer (abbreviation: BRCA or BC, Figure 2B).