Through anti-tumor experiments in vivo and in vitro, we found that MT-loaded PP5 scaffold has better anti-tumor effect than melatonin alone, the cell migration in transwell assay showed that the MG-3 cell was inhabited of in the MT and MT-loaded PP5 group in Figrue 6, more interestingly, the MT-loaded PP5 group has more effective anti-tumor ability, the weight of tumor in MT-loaded PP5 group was smaller than other two groups, in order to further analyze this phenomenon, we detected autophagy-related markers, including LC3 and P62. This evidence concerns the gene MAP1LC3A and neoplasm.