In summary, our data show that NK-92/5.28.z cells exhibit high cytotoxicity in r/r ErbB2-positive 2D patient-derived tumor organoid RMS cells and 3D aRMS tumor spheroid models in vitro, which was reproduced and confirmed in an experimental metastasis model in NSG mice in vivo not representing the adoptive immunity of bystander cells. Here, ERBB2 is linked to neoplasm.