By qRT-PCR and western blotting, we found that compared with normal hepatocytes THLE-2, mRNA and protein of LPCAT1 were substantially overexpressed in HCC cells (MHCC97-H, HepG2, and HCCLM3) (all p < 0.05, Figures 10A, B). This evidence concerns the gene LPCAT1 and hepatocellular carcinoma.