This study identified the upregulation of Sestrin2 in an asthma rat model and cell model and revealed that the increased Sestrin2 promoted the growth, migration, and calcium flow of ASMCs in the asthma group, showed the regulation of Sestrin2 by miR-182, and elucidated the involvement of the AMPK/mTOR pathway in the role of Sestrin2 in ASMCs of asthma. Here, MTOR is linked to asthma.