To interrogate the mechanism of receptor activation, ST6GAL1 was overexpressed (OE) in the OV4 ovarian cancer line, which lacks endogenous ST6GAL1, or knocked-down (KD) in OVCAR-3 and OVCAR-5 ovarian cancer cells, which have high levels of ST6GAL1. The gene discussed is ST6GAL1; the disease is ovarian cancer.