FN1 and keloid: Analysis of ECM anisotropy using a high throughput image analysis algorithm, Alignment by Fourier Transform (AFT) [13,17] (Fig. 1B,C and Supplemental Fig. 1B,C), revealed that keloid patient fibroblasts (KDF) produced a highly aligned fibronectin and collagen matrix in culture compared to normal dermal fibroblasts (NDF).