In the in vivo study, we detected a gross lower expression level of CD68 and ARG1 (macrophage), Aggrecan (chondrocyte), OPN (osteoblast), ADIPQ (adipocytes), CD34 (mesenchymal cells), and LUM (fibroblasts) in the FAM3A-overexpressing aneurysm tissues compared with the matched Ad-sham tissues (Fig. 4a). This evidence concerns the gene SPP1 and aneurysm.