GRN and metastatic malignant neoplasm in the lymph nodes: According to the method of independent sample t test in medical statistics, compared to the group without lymph node metastasis, the PGRN mRNA expression level and PGRN protein expression level in the group with lymph node metastasis were significantly increased by 75% at mRNA expression level (2−ΔΔCt: 28.925 ± 5.867 vs. 11.081 ± 4.222; P < 0.05) and increased by 161% at relative protein expression level (1.127 ± 0.143 vs. 0.642 ± 0.174; P < 0.05).