Since the molecular weight of FAT4 full-length protein is 543 kDa, we used the deficient Cas9-synergistic activation mediator (dCas9-SAM) technology to transfect ME180 cervical cancer cell lines with short guide RNA (sgRNA) targeting the FAT4 promoter region and endogenously promote FAT4 expression. The gene discussed is FAT4; the disease is cervical carcinoma.