FAT4 and cervical cancer: FAT4 expression was detected in six cervical cancer cell lines using immunofluorescence and immunoblotting, with FAT4 expression being lower in ME180, C33A, and U14 cells and higher in SiHa, Caski, and Hela cells (Fig. 1H), but cell membrane localization of FAT4 was not observed in any of the above cell lines (Fig. 1I).