ALK and non-small cell lung carcinoma: This study collected the cases of advanced NSCLC patients with ALK fusion gene positive that were confirmed by both of the method of NGS and IHC to analyze the characteristics of ALK fusion gene subtypes, abundance et al. We further analyzed the relationships between different molecular types of ALK fusion gene, clinicopathological characteristics and prognosis, hoping to reveal the mutation status of ALK fusion gene positive advanced NSCLC patients.