The GO results indicated that combination DEGs were significantly involved in apoptotic process GO term in both HCC cell lines and KEGG results showed that combination DEGs were significantly involved in cancer-related different signaling pathways such as apoptosis, ferroptosis, cellular senescence, cell cycle, p53 signaling pathway, MAPK signaling pathway, IL-7 signaling pathway and TNF signaling pathway. The gene discussed is TNF; the disease is cancer.